17.What information is critical to the success of polymerase chain reaction (PCR) itself?
(A) The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
(B)The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the plasmid
where the amplified DNA fragment will be cloned must be known.
(C) The complete DNA sequence of the DNA to be amplified must be known.
(D) The DNA sequence of the ends of the DNA to be amplified must be known.
(E) The Tm of primers must be known.