問題詳情
28. When doing PCR to amplify a DNA fragment using mouse genomic DNA as the template, which of the following primer pair is the best choice? Forward primers: i. ACGTCTGCCAGGCTG, 15bp, GC content=67%, Tm=57℃
ii. AGCTTGGGTTAATGCCAGGC, 20bp, GC content-55%, Tm=60℃
iii. AGCCCCATTGCTATAAGGGGAG, 22bp, GC content=55%, Tm=59℃