【etrnya】評論
the purpose of detecting antigen-specific antibody-secreting cells (ASC) in a B cell ELISPOT assay, which was a modification of a traditional sandwich ELISA immunoassay.The ELISPOT assay has since been more widely adopted for the identification and enumeration of cytokine-producing cells at the single cell level, but is still used for detection of ASC.
【李嘉哲】評論
ELISPOT是ELISA的延伸應用 孔盤被覆待測物的抗體,再加入可分泌該待測物的細胞群,後續如同ELSIA的步驟,最後計算孔盤中著色斑點數量來決定加入的細胞懸浮液含多少可分泌待測物的細胞群