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• 3. The E. coli recombinant plasmid pBR322 has been widely utilized in genetic engineeringexperiments
• 4. Which of the following statements regarding plasmid cloning vectors is correct?(A) Circular plasm
• 5. When considering the nature of genetic regulation in eukaryotes, one often comes across theterm &
• 6. Although the expression of most genes is tightly regulated, some genes are expressed at roughlyco
• 7. The relationship between DNA fragment size and mobility in an agarose gel is best described asa(n
• 8. The Sanger or dideoxy method of nucleotide sequence determination(A) relies on sequence-specific
• 9. Which of the following methods can be used to detect the gene activity?(A) primer extension (B) N
• 10. Which of the following is most likely to lead to a loss-of-function of a gene?(A) A change from
• 11. An E. coli cloning vector would usually contain all of the following EXCEPT:(A) an E.coli ori(B)
• 12. The target sequence used in PCR is:(A) the sequence the Taq polymerase recognizes in order to bi
• 13. In the Southern blotting procedure(A) Labeled probe DNA is used to visualize target DNA bound to
• 14. Gene regulation in both prokaryotes and eukaryotes is achieved by controlling which process?(A)
• 15. The standard Watson-Crick model of DNA favored at high relative humidity and in solution is(A) Z
• 16. Restriction enzymes are found in virtually all bacterial species, and their sequence specificiti
• 17. What are the functions of ammonium persulfate (APS), TEMED and SDS used inpolyacrylamide gel ele
• 18. Which of the following does not apply to the construction or use of a DNA library?(A) Specialize
• 19. Which of the following characteristics are the principles of separation of proteins in denaturin
• 20. The ribosome is involved in all of the following except(A) aminoacylation of tRNA (B) binding of
• 21. The newly synthesized DNA with addition of nucleotides moving in the same direction as thereplic
• 22. Assume that you were given a vial containing 0.05μg/μl of DNA and you are asked to set up areact
• 23. A certain bacterial mRNA is known to represent only one gene and to contain about 800nucleotides
• 24. A messenger ribonucleic acid is 336 nucleotides long, including the initiator and terminationcod
• 25. Assuming that the average amino acid residue contributes 110 to the peptide molecular weight,wha
• 1.預估、募集資金以支援企業需求，並將財務風險控制在可接受的範圍內，是下列哪一項企業功能所執行的活動？(A)資源管理 (B)財務管理(C)行銷管理 (D)作業管理
• 2. 屋內配線設計圖無熔線開關之符號為 (A) (B) (C) (D)