問題詳情

78. In DNA sequencing by the Sanger (dideoxy) method:
(A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesisof complementary strands.
(B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are thenseparated by electrophoresis.
(C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence.
(D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gsoccur in the template strands.
(E) the template DNA strand is radioactive.

參考答案

答案:D
難度:計算中-1
書單:沒有書單,新增