45. Which of the following sequence of the steps is regular for Western Blotting Assay that is widely used for detection of thespecific protein in a tissue?1. Tissues are broken down using a homogenizer or by sonication.  2. The protein is probed with an addition of the primary antibody. 3. The protein is transferred to a PVDF membrane.  4. Add secondary antibody conjugated with peroxidase.  5. The proteins of the tissues are separated using gel electrophoresis.6. The protein is detected with an addition of a peroxidase substrate.
(A) 123456
(B) 153246
(C) 152346
(D) 154623
(E) 124653