27. Which of the following methods concerning high-performance liquid chromatography (HPLC) areINCORRECT?
(A) Ion-pair chromatography is powerful for the separation of metal ions due to the addition of ion-pair reagents.
(B) Size-exclusion chromatography is useful for the separation of large-sized macromolecules, butit suffers from limited resolution and long analysis time
(C) In reversed-phase HPLC, polar analytes elute carlier than nonpolar ones.
(D) In ion-exchange chromatography, ion exchangers bind preferably ions of higher charge andincreased hydrated radius