16. About mutation, which of the following stateme ent is TRUE?
(A) Silent mutations usually do not have obser rvable effects onphenotypes becaus se they are in-frame mutations
(B) Missense mutations change a codon into a stop codonleading to a trunc cated protein
(C) Nonsense mutation is a point mutation in which a singlenucleotide change resulted in a a change in an amino acid
(D) Insertions and deletions are additions or losses ofnucleotide pairs in a gene that alter the reading frameproducing out-of-frame mutations