問題詳情

18.Is sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide
end? Do the labeled nucleotides lack the 2' or 3' hydroxyI group?
(A) 3' end； 2' hydroxyl group only
(B) 3' end； 3' hydroxyl group only
(C) 5' end； 2' hydroxyl group only
(D) 5' end； 3' hydroxyl group only
(E) 3' end； both hydroxyl groups

參考答案

答案：99

統計：A:0,B:0,C:0,D:0,E:0

難度：計算中

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In Sanger sequencing, does each labeled DNA molecule have a fluorescently labeled dideoxyribonucleotide end? Do the labeled nucleotides lack the 2' or 3' hydroxyI group? (E) 3' end； both hydroxyl groupsThe dideoxyribonucleotides terminate the chains because they lack the 3′ hydroxyl group required for further polymerization. Additionally, all DNA nucleotides lack the 2′ hydroxyl group.