【評論主題】55 下列何種試驗無法用來區別 Bacillus anthracis 與 Bacillus cereus?(A)運動性 (B)在 sheep blood agar 的溶血性 (C) lecithina

【評論內容】Lecithinase test or Nagler’s reaction is a biochemical test used to identify organisms which liberate phospholipases (lecithinases) e.g. Clostridium perfringens. The alpha (α) toxin of C. perfringens has phospholipase activity and hence, helps in differentiation of C. perfringens from other Clostridium spp that also produce lecithinase (C.baratti, C.absonum, C.bifermantans, C.sordelli and C.novyi)  by neutralization of lecithin c activity by an antitoxin.Bacillius cereus also exhibit strong lecithinase activity but can be differentiated by its strong hemolytic property on sheep blood agar a...

【評論主題】23 下列各種培養基均可用於分離 Bordetella pertussis,惟何者例外?(A) Bordet-Gengou(BG)agar (B) chocolate agar (C) cyclode

【評論內容】

分離Bordetella pertussis

BG培養基(bordet gengou agar)-較常使用Regan-Lowe charcoal培養基

CSM(cyclodextrin solid medium)

【評論主題】47 從病人腦脊髓液培養出一種酵母菌,菌落黏稠,菌體大小不一,尿素酶陽性,疑為 Cryptococcusneoformans,下列何者可做為鑑定此菌之主要依據?(A) Nitrate reductio

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Cryptococcus neoformans具有產生尿素酶的能力、抗生素的抵抗性,並有phenoloxidase活性,能將兒茶酚胺前趨物 (catecholamine precursors) 製成黑色素 (melanin),因此培養基若含有兒茶酚胺,如birdseed agar(Niger seed agar),其生長培育出來的菌落具有黑色素,可加以確認。