用戶【榜上有Ling】點評問題和點評內容

【評論主題】100.下列何者為桑格定序法(Sanger sequencing method)的關鍵分子?(A)1', 2'-雙脫氧核苷酸(1', 2'-dideoxyrib

【評論內容】

課本敘述:Sanger sequencing:Each ddNTP is derivatized with a fluorescent dye(螢光染色), each of a different color. Thus, each fragment has a distinct color based upon the identity of the ddNTP that terminated the sequencing reaction. This allows all four reaction mixtures to be resolved in one lane of a sequencing gel, permitting analysis of far more DNA in one sequencing operation. The gel is scanned fluorometrically, and a computer reads the DNA sequence directly from the resulting pattern of differently colored peaks.(可以從不同的螢光顏色結果判斷DNA序列)附上熟悉的圖片

【評論主題】97.在細胞凋亡程序中的caspases為一種cysteine protease,其切割受質蛋白的位點位於下列何種胺基酸的C端?(A)絲胺酸(serine) (B)胱胺酸(cysteine) (C)天

【評論內容】caspases 胱天蛋白酶:1.參與細胞凋亡(A family of proteases involved in apoptosis)。2.又叫做cysteine aspartate proteases,含有cysteine 並攻擊aspartate(have an active site cysteine and attack bonds containing an asp residue)。不負責任記法:caspases藏有asp

【評論主題】80.下列何種酵素與尿酸堆積及痛風發生最為相關?(A)adenine phosphoribosyltransferase (B)5-phospho-α-D-ribosyl-1-pyrophosphat

【評論內容】

(A)adenine phosphoribosyltransferase=APRT(B)5-phospho-α-D-ribosyl-1-pyrophosphate synthetase=PRPP,和痛風有關。