12.Real-time polymerase chain reaction (RT-PCR) is a very ry sensitive method, allowing the detection of extremelylow amounts of nucleic acid present in the samples. Which statements conceming RT-qPCR is/areCORRECT?
(A) The method relies on the use of fluoresc cent dye that incorporates into DNA for quantification
(B) This method can be used to detect COVID-19 virus a nasal swab
(C) This method is only applicable for quantifying RNA which is reversibly transcribed into DNA but notDNA per se
(D) Like regular PCR, RT:PCR detect end-point PCR products
(E)Like regular PCR, the polymerase for RT-PCR is isolated from bacteria of hot spring