45. An analysis of chromosomal DNA, using the Southern blot technique, involves the following five major steps:①Autoradiography； ②Blotting； ③ Cleavage；④Electrophoresis； ⑤Hybridization；
Which of the following sequences of steps best illustrates this technique?
(A)①②③④⑤
(B) ①③②④⑤
(C) ③⑤②④①
(D) ③②⑤④①
(E) ③④②⑤①